Evaluation of the Vitamin B12 Deficiency in Megaloblastic Anemia in Tertiary Care Hospital, Lahore.
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Abstract
Background: Megaloblastic anemia is a hematologic disorder characterized by the presence of large, immature red blood cells due to a deficiency in vitamin B12 or folate. It remains a significant public health issue globally, affecting various populations across different age groups and dietary habits.
Objective: The objective of this study was to evaluate the prevalence and implications of vitamin B12 deficiency in the development of megaloblastic anemia in a tertiary care hospital in Lahore.
Methods: A cross-sectional analysis was conducted on 85 patients over a period of four months. Ethical approval was secured in line with the Declaration of Helsinki. Participants included both genders with hemoglobin levels <10 g/dl or mean corpuscular volume >95 fL, excluding pregnant women and individuals with primary diseases such as hepatic, renal, or cancerous conditions. Verbal consent was obtained before blood sample collection. CBC parameters were analyzed using a Mindray hematology analyzer, and vitamin B12 levels were measured via ELISA. Statistical evaluation was performed using SPSS version 25.0.
Results: The mean hemoglobin levels for males and females were 10.06 ± 3.10 g/dl and 9.61 ± 3.25 g/dl, respectively. The mean MCV for males was 99.94 ± 6.63 fL, and for females, it was 100.25 ± 7.90 fL. Vitamin B12 levels averaged at 489.92 ± 468.10 pg/ml for males and 440.19 ± 419.47 pg/ml for females. Of the patients, 47.05% had normal B12 levels, 25.88% were deficient, and 27.05% had high levels of B12.
Conclusion: Vitamin B12 deficiency was prevalent among patients with megaloblastic anemia and was independent of gender or age. The findings suggest a strong need for enhanced nutritional awareness and screening practices to identify and treat this deficiency early in at-risk populations.
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References
Khanduri, U. and A. Sharma, Megaloblastic anaemia: prevalence and causative factors. National Medical Journal of India, 2007. 20(4): p. 172-175.
Devrajani, B.R., et al., Helicobacter pylori: a cause of vitamin B12 deficiency (a hospital based multidisciplinary study). World Applied Sciences Journal, 2011. 12(9): p. 1378-81.
Soofi, S., et al., Prevalence and possible factors associated with anaemia, and vitamin B 12 and folate deficiencies in women of reproductive age in Pakistan: analysis of national-level secondary survey data. BMJ open, 2017. 7(12): p. e018007.
Hsu, H.-Y.L., Realization Right to Health in the Context of Pharmaceutical under International Law. 2009.
Iqbal, W., et al., Aetiological breakup in 208 cases of pancytopenia. J Rawal Med Coll, 2001. 5(1): p. 7-9.
Gibson, R.S., Principles of nutritional assessment. 2005: Oxford university press, USA.
Warren, M.J., et al., Vitamin B 12: Biosynthesis of the corrin ring. Tetrapyrroles: Birth, Life and Death, 2009: p. 286-299.
Gille, D. and A. Schmid, Vitamin B12 in meat and dairy products. Nutrition reviews, 2015. 73(2): p. 106-115.
Khajuria, A. and R. Das, Prevalence of megaloblastic anaemia and its causative factors in a tertiary care centre at Western India. Santosh University Journal of Health Sciences, 2022. 8(2): p. 141.
Nathan, D.G. and F.A. Oski, Nathan and Oski's hematology of infancy and childhood. (No Title), 2003.
Asif Hussain, M., et al., Bone marrow aspiration findings in a tertiary care hospital of Peshawar. 2015.
Jan, A.Z., Z. Gul, and F. Liaqat, Megaloblastic anemia and pattern of its presentation in children. Gomal Journal of Medical Sciences, 2016. 14(2).
Štifter, S., et al., Combined evaluation of bone marrow aspirate and biopsy is superior in the prognosis of multiple myeloma. Diagnostic pathology, 2010. 5(1): p. 1-7.
Sharourou, A.S.A., et al., Anemia: Its prevalence, causes, and management. The Egyptian Journal of Hospital Medicine, 2018. 70(10): p. 1877-1879.
Wu, Q., et al., Mechanism of megaloblastic anemia combined with hemolysis. Bioengineered, 2021. 12(1): p. 6703-6712.
Unnikrishnan, V., et al., Clinico-aetiologic profile of macrocytic anemias with special reference to megaloblastic anemia. Indian Journal of Hematology and Blood Transfusion, 2008. 24: p. 155-165.
Wafaa, H., et al., Is Vitamin B12 Level a Reliable Predictor of Psychosis Severity in Male Patients with Megaloblastic Anemia at a Single Tertiary Hospital? International Journal of General Medicine, 2022: p. 7657-7668.
Beckman Coulter, M., Analyzer Operator's Guide.(1992, January 21). PN 4235935J.
Manual, I. and I. Bio-Rad Quantaphase, Folate Radioassay Kit. Hercules, CA: Bio-Rad Laboratories, 1993.
Langhani, S.K., PREVALENCE OF SERUM FOLIC ACID DEFICIENCY AND DEMONSTRATION OF HAEMATOLOGICAL PARAMETERS AMONG POPULATION OF THARPARKAR SINDH, PAKISTAN. Journal of Peoples University of Medical & Health Sciences Nawabshah.(JPUMHS), 2021. 11(1): p. 105-109.
Mahajan, S.K. and S.C. Aundhakar, A study of the prevalence of serum vitamin B12 and folic acid deficiency in Western Maharashtra. Journal of family medicine and primary care, 2015. 4(1): p. 64.
Riaz, H., M.A. Shah, and I. Ullah, FREQUENCY OF MEGALOBLASTIC ANAEMIA AMONG PATIENTS WITH VARIOUS HAEMATOLOGICAL DISORDERS AFTER BONE MARROW EXAMINATION IN PESHAWAR (A TERTIARY-CARE HOSPITAL STUDY). KJMS, 2018. 11(2): p. 318.
Rehman, S., et al., Vitamin B12 deficiency among patients with megaloblastic anemia. Journal of Medical Sciences, 2019. 27(2): p. 103-106.
Arshad, M., et al., Iron deficiency anemia and megaloblastic anemia in obese patients. Rom J Intern Med, 2017. 55(1): p. 3-7.
Rana, K.S., et al., Subacute combined degeneration of spinal cord due to nutritional vitamin B12 deficiency in early childhood. Journal of Pediatric Neurology, 2012. 10(02): p. 129-132.