Cannabinoid Detection in Hemp Seed Oil, Hemp Seed, and Blood of Rohu (Labeo rohita) Following 50-Day Dietary Supplementation
DOI:
https://doi.org/10.61919/jhrr.v3i2.2040Keywords:
Cannabis sativa; cannabinoids; hemp seed; hemp seed oil; Labeo rohita; gas chromatography-mass spectrometry; dietary supplementation.Abstract
Background: Hemp (Cannabis sativa L.) seed and seed oil are increasingly considered as potential feed ingredients because of their nutritional composition. However, the presence of cannabinoids in hemp-derived ingredients raises questions regarding their possible detection in animals following dietary exposure. This study evaluated cannabinoids in hemp seed oil and whole hemp seed and investigated their detection in the blood of Rohu (Labeo rohita) following dietary supplementation for 50 days. Methods: Cannabinoids were analyzed using gas chromatography-mass spectrometry (GC-MS). Seven experimental diets were used, comprising a basal control diet, three diets supplemented with hemp seed oil at 3%, 6%, and 9%, and three diets supplemented with hemp seed at 5%, 10%, and 15%. Rohu fingerlings received the respective diets for 50 days. At the end of the feeding period, blood samples were collected and analyzed for the targeted cannabinoid compounds. Results: Three target compounds were detected in hemp seed oil: delta-9-tetrahydrocannabinol (Δ9-THC; 9.77 ± 0.66%), cannabinol (CBN; 9.30 ± 0.43%), and resorcinol (22.96 ± 0.87%). In whole hemp seed, cannabidiol (CBD) was the only target cannabinoid detected, at 0.06%. None of the targeted cannabinoids were detected in blood samples from the control group or from Rohu fed diets supplemented with hemp seed oil or hemp seed. Conclusion: Under the conditions of this 50-day feeding experiment, the targeted cannabinoids were not detected in the blood of Rohu receiving diets containing up to 9% hemp seed oil or 15% hemp seed. These findings describe cannabinoid detection in the tested hemp-derived feed ingredients and their absence from Rohu blood within the analytical conditions used in this study. Keywords: Cannabis sativa; cannabinoids; hemp seed; hemp seed oil; Labeo rohita; gas chromatography-mass spectrometry; dietary supplementation.
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