Oxidative Stress, Hematological and Histopathological Alterations Recovery by Methanolic Extract of Celtis Occidentalis L. Leaves in Paracetamol-Induced Hepatic Injury in Rabbit
Recovery of Paracetamol-Induced Hepatic Injury by Celtis Occidentalis Extract
DOI:
https://doi.org/10.61919/jhrr.v4i3.1487Keywords:
C.occidentalis, Oxidative stress, Hepatic injury, Silymarin, Antioxidant enzymes, Liver biomarkers, Hematology, HistopathologyAbstract
Background: Paracetamol overdose is a leading cause of hepatotoxicity, resulting in oxidative stress and liver damage. Exploring natural therapeutic agents like Celtis occidentalis for their hepatoprotective effects is essential to mitigate these effects.
Objective: To evaluate the hepatoprotective, antioxidant, and nephroprotective effects of methanolic extract of Celtis occidentalis leaves in paracetamol-induced hepatic injury in rabbits.
Methods: The study involved 40 male rabbits divided into eight groups, including control, paracetamol (2 g/kg), and various doses of Celtis occidentalis extract (100, 200, and 300 mg/kg) with or without silymarin (50 mg/kg). The extract's antioxidant potential was assessed using DPPH and ABTS assays. Hepatic and renal function tests, lipid profiles, and hematological parameters were measured, and histopathological evaluations of liver tissues were conducted.
Results: The extract showed significant antioxidant activity, with 87.7% DPPH and 89.8% ABTS scavenging at 1000 µg/mL. It reduced ALT, AST, ALP, and bilirubin levels significantly (P<0.05). High dose extract normalized lipid profiles and improved hematological parameters compared to paracetamol alone.
Conclusion: Celtis occidentalis extract exhibited potent hepatoprotective effects, normalizing liver function and mitigating oxidative stress, suggesting its potential use in human healthcare for liver protection.
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References
Gunnell D, Murray V, Hawton K. Use of Paracetamol (Acetaminophen) for Suicide and Nonfatal Poisoning: Worldwide Patterns of Use and Misuse. Suicide Life Threat Behav. 2000;30(4):313-326.
Khoursandi L, Ourazizadeh M. Protective Effect of Curcuma Longa Extract on Acetaminophen Induced Nephrotoxicity in Mice. 2008.
Wan XY, Luo M, Li XD, He P. Hepatoprotective and Anti-Hepatocarcinogenic Effects of Glycyrrhizin and Matrine. Chem Biol Interact. 2009;181(1):15-19.
Rui L. Energy Metabolism in the Liver. Compr Physiol. 2014;4(1):177.
Thorne AM, Ubbink R, Brüggenwirth IM, Nijsten MW, Porte RJ, de Meijer VE. Hyperthermia-Induced Changes in Liver Physiology and Metabolism: A Rationale for Hyperthermic Machine Perfusion. Am J Physiol Gastrointest Liver Physiol. 2020;319(1).
Rehman JU, Saqib NU, Akhtar N, Jamshaid M, Asif HM, Sultana S, Rehman RU. Hepatoprotective Activity of Aqueous-Methanolic Extract of Suaeda Fruticosa in Paracetamol-Induced Hepatotoxicity in Rabbits. Bangladesh J Pharmacol. 2013;8(4):378-381.
Kassem FF, Alqasoumi SI, Sallam SM, Bekhit AA, Al-Shaer N, Farraj A. Evaluation of the Hepatoprotective, Nephroprotective and Anti-Malarial Activities of Different Parts of Bauhinia Purpurae and Tipuana Speciosa Grown in Egypt. J Med Plant Res. 2013;7:1190-1200.
Jaeschke H, Ramachandran A. The Role of Oxidant Stress in Acetaminophen-Induced Liver Injury. Curr Opin Toxicol. 2020;20:9-14.
Nazir N, Zahoor M, Nisar M. A Review on Traditional Uses and Pharmacological Importance of Genus Elaeagnus Species. Bot Rev. 2020;86:247-280.
Nazir N, Zahoor M, Nisar M, Karim N, Latif A, Ahmad S, Uddin Z. Evaluation of Neuroprotective and Anti-Amnesic Effects of Elaeagnus Umbellata Thunb. On Scopolamine-Induced Memory Impairment in Mice. BMC Complement Med Ther. 2020;20:1-17.
Vargas-Pozada EE, Muriel P. Herbal Medicines for the Liver: From Bench to Bedside. Eur J Gastroenterol Hepatol. 2020;32(2):148-158.
Keeler HL. Our Native Trees and How to Identify Them: A Popular Study of Their Habits and Their Peculiarities. Scribner's; 1915.
Kazancioglu S, Yilmaz FM, Bastug A, Sakalli A, Ozbay BO, Buyuktarakci C, Bodur H, Yilmaz G. Lymphocyte Subset Alteration and Monocyte CD4 Expression Reduction in Patients with Severe COVID-19. Viral Immunol. 2021;34(5):342-351.
Jalil-ur-Rehman JUR, Akhtar N, Khan MY, Ahmad KA, Ahmad MA, Sultana S, Asif HM. Phytochemical Screening and Hepatoprotective Effect of Alhagi Maurorum Boiss (Leguminosae) Against Paracetamol-Induced Hepatotoxicity in Rabbits.
Wagner C, De Gezelle J, Komarnytsky S. Celtic Provenance in Traditional Herbal Medicine of Medieval Wales and Classical Antiquity. Front Pharmacol. 2020;11:105.
Lauriault J. Identification Guide to the Trees of Canada. National Museum of Natural Sciences; 1989.
Moerman DE. Native American Ethnobotany. Timber Press; 1998.
Hillis W, Swain T. The Phenolic Constituents of Prunus Domestica. II.—The Analysis of Tissues of the Victoria Plum Tree. J Sci Food Agric. 1959;10(2):135-144.
Kiranmai M, Kumar CM, Mohammed I. Comparison of Total Flavanoid Content of Azadirachta Indica Root Bark Extracts Prepared by Different Methods of Extraction. Res J Pharm Biol Chem Sci. 2011;2(3):254-261.
Brand-Williams W, Cuvelier ME, Berset C. Use of a Free Radical Method to Evaluate Antioxidant Activity. LWT-Food Sci Technol. 1995;28(1):25-30.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radic Biol Med. 1999;26(9-10):1231-1237.
Nazir N, Nisar M, Zahoor M, Uddin F, Ullah S, Ullah R, Ansari SA, Mahmood HM, Bari A, Alobaid A. Phytochemical Analysis, in Vitro Anticholinesterase, Antioxidant Activity and in Vivo Nootropic Effect of Ferula Ammoniacum (Dorema Ammoniacum) D. Don. in Scopolamine-Induced Memory Impairment in Mice. Brain Sci. 2021;11(2):259.
Aebi H. Catalase in Vitro. Methods Enzymol. 1984;105:121-126.
Nishikimi M, Rao NA, Yagi K. The Occurrence of Superoxide Anion in the Reaction of Reduced Phenazine Methosulfate and Molecular Oxygen. Biochem Biophys Res Commun. 1972;46(2):849-854.
Beutler E, Duron O, Kelly BM. Improved Method for the Determination of Blood Glutathione. J Lab Clin Med. 1963;61:882-888.
Abu-Serie MM, Habashy NH. Vitis Vinifera Polyphenols from Seedless Black Fruit Act Synergistically to Suppress Hepatotoxicity by Targeting Necroptosis and Pro-Fibrotic Mediators. Sci Rep. 2020;10(1):2452.
Minsart C, Rorive S, Lemmers A, Quertinmont E, Gustot T. N-Acetylcysteine and Glycyrrhizin Combination: Benefit Outcome in a Murine Model of Acetaminophen-Induced Liver Failure. World J Hepatol. 2020;12(9):596.
Owens C, Belcher R. A Colorimetric Micro-Method for the Determination of Glutathione. Biochem J. 1965;94(3):705.
El-Deberky D, Rizk M, Elsayd F, Amin A, El-Mahmoudy A. Protective Potential of Cynara Scolymus Extract in Thioacetamide Model of Hepatic Injury in Rats. Bionatura. 2021;6(2):1792-1802.
Martins JL, Rodrigues OR, de Sousa FB, Fajemiroye JO, Galdino PM, Florentino IF, Costa EA. Medicinal Species with Gastroprotective Activity Found in the Brazilian Cerrado. Fundam Clin Pharmacol. 2015;29(3):238-251.
Ighodaro O, Akinloye O. First Line Defence Antioxidants-Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPX): Their Fundamental Role in the Entire Antioxidant Defence Grid. Alex J Med. 2018;54(4):287-293.
Łukaszewicz-Hussain A, Moniuszko-Jakoniuk J, Rogalska J. Assessment of Lipid Peroxidation in Rat Tissues in Subacute Chlorfenvinphos Administration. Pol J Environ Stud. 2007;16(2).
Mian P, Van Den Anker JN, van Calsteren K, Annaert P, Tibboel D, Pfister M, Allegaert K, Dallmann A. Physiologically Based Pharmacokinetic Modeling to Characterize Acetaminophen Pharmacokinetics and N-Acetyl-p-Benzoquinone Imine (NAPQI) Formation in Non-Pregnant and Pregnant Women. Clin Pharmacokinet. 2020;59:97-110.
Deepthi A, Prasad C, Prasad BR. Study of Dimorphic Anemia in Adults with Reference to Basic Etiology. Indian J Pathol Oncol. 2018;5:61-66.
Li J, Jiang H, Wu P, Li S, Han B, Yang Q, Wang X, Han B, Deng N, Qu B, Zhang Z. Toxicological Effects of Deltamethrin on Quail Cerebrum: Weakened Antioxidant Defense and Enhanced Apoptosis. Environ Pollut. 2021;286:117319.
Hwang BY, Chai HB, Kardono LB, Riswan S, Farnsworth NR, Cordell GA, Pezzuto JM, Kinghorn AD. Cytotoxic Triterpenes from the Twigs of Celtis Philippinensis. Phytochemistry. 2003;62(2):197-201.
Uma N, Fakurazi S, Hairuszah I. Moringa Oleifera Enhances Liver Antioxidant Status via Elevation of Antioxidant Enzymes Activity and Counteracts Paracetamol-Induced Hepatotoxicity. Malays J Nutr. 2010;16(2).
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