Evaluation of Phytochemicals and Antibacterial Usefulness of Citrus Limon & Cicer Arietinum in Synergistic Effect with Antibiotics Against Clinically Important Bacteria

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Aamir Sattar
Afshan Syed Abbas
Sajida Naseem
Madiha Mumtaz
Asad Shabbir
Saima Shahzad Mirza


Background: The emergence of antibiotic resistance necessitates exploring alternative treatments, including natural plant extracts. Lemon juice (Citrus limon) and chickpea (Cicer arietinum) seed aqueous extract, known for their medicinal properties, are investigated for their potential antibacterial effects.

Objective: This study aimed to assess the antibacterial efficacy of lemon juice and chickpea seed aqueous extract, individually and in combination with antibiotics, against various pathogenic bacterial strains.

Methods: Aqueous and ethanolic extracts of C. limon and C. arietinum were prepared. Antibacterial activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa, sourced from the University of Education, Lahore, was assessed using the disc diffusion method. Extract concentrations of 5%, 10%, 20%, and 40% were tested. Phytochemical analysis identified active compounds in the extracts. Antibacterial efficacy was quantified by measuring inhibition zones in millimeters.

Results: Lemon juice showed dose-dependent antibacterial activity with inhibition zones up to 19.8±0.088 mm at 40% concentration. Chickpea extract was effective against S. aureus with a maximum inhibition of 14±0.577 mm at 40%. Combined treatments enhanced antibacterial effects, particularly against S. aureus, with a combination of lemon juice, chickpea extract, and antibiotics yielding an inhibition zone of 20.1±0.058 mm at the highest concentration. In contrast, chickpea extract alone showed no activity against E. coli, K. pneumoniae, or P. aeruginosa.

Conclusion: The study confirms the significant antibacterial potential of lemon juice across multiple bacterial strains and the specific efficacy of chickpea extract against S. aureus. The combination of these extracts with antibiotics suggests a promising avenue for augmenting antibacterial treatment strategies.

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How to Cite
Sattar, A., Abbas, A. S., Naseem , S., Mumtaz, M., Shabbir, A., & Mirza, S. S. (2024). Evaluation of Phytochemicals and Antibacterial Usefulness of Citrus Limon & Cicer Arietinum in Synergistic Effect with Antibiotics Against Clinically Important Bacteria. Journal of Health and Rehabilitation Research, 4(1), 244–249. https://doi.org/10.61919/jhrr.v4i1.174
Author Biography

Asad Shabbir, University of Sialkot Pakistan.

Department of Zoology.


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