Association of HLA-G Polymorphism at rs66554220 14-bp Insertion/Deletion Variant with HPV-Related Cervical Cancer: A Cross-Sectional Study HLA-G Polymorphism and HPV-Related Cervical Cancer
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Abstract
Background: Cervical cancer is a major cause of cancer-related morbidity and mortality in women globally, predominantly caused by persistent infection with high-risk human papillomavirus (HPV) types. Genetic factors, including HLA-G polymorphisms, may contribute to HPV-related cervical cancer susceptibility.
Objective: To evaluate the association of HLA-G polymorphism at rs66554220 with HPV-related cervical cancer in women presenting to a gynecology OPD.
Methods: A cross-sectional study was conducted at Ziauddin Hospital from January 2023 to May 2024. Cervical smear samples were obtained from 41 women, and DNA was extracted for genotyping using the QIAamp DNA Mini Kit. HPV detection was performed using PCR with L1-specific primers, and HLA-G polymorphism at rs66554220 was analyzed using PCR and Sanger sequencing. Statistical analysis was performed using SPSS version 25, with significance set at p < 0.05.
Results: The 14-bp deletion variant was found in 26.8% of participants, and 19.5% tested positive for HPV. The deletion polymorphism significantly increased HPV-related cervical cancer risk (χ² = 27.1, OR = 12.7, 95% CI: 3.14–51.56, p < 0.001).
Conclusion: The HLA-G polymorphism at rs66554220 is significantly associated with HPV-related cervical cancer, suggesting its potential role in cervical cancer screening.
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