Estimation of Trace and Toxic Metals from Cannabis Sativa Plant in District, Kech Baluchistan, Pakistan
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Abstract
Background: Cannabis sativa, a plant indigenous to the Kech district in Baluchistan, Pakistan, is traditionally used for medicinal purposes. Understanding its trace and toxic metal content is crucial for evaluating its safety and therapeutic potential.
Objective: The primary aim of this study is to estimate the levels of trace and toxic metals in various parts of Cannabis sativa plants grown in the Kech district and assess the potential health risks associated with their consumption.
Methods: The leaves, stems, and roots of Cannabis sativa were analyzed for metals including Iron (Fe), Copper (Cu), Zinc (Zn), Potassium (K), Magnesium (Mg), Calcium (Ca), Sodium (Na), Cadmium (Cd), Lead (Pb), and Nickel (Co). Flame Atomic Absorption Spectroscopy was employed to quantify the metal content.
Results: The plant exhibited high levels of Fe (20.34 ppm in leaves), Zn (24.14 ppm in leaves), Ca (9.29 ppm in leaves), Cu (14.40 ppm in leaves), K (10.56 ppm in leaves), Mg (13.47 ppm in leaves), and Na (12.25 ppm in leaves). Conversely, lower concentrations of Cd (0.65 ppm in leaves), Co (2.29 ppm in leaves), As (0.53 ppm in leaves), and Pb (1.60 ppm in leaves) were detected. The leaves contained higher concentrations of these metals compared to the stems and roots.
Conclusion: The presence of beneficial trace metals like Fe, Cu, Zn, and Ca in significant amounts suggests Cannabis sativa's potential for treating diseases related to deficiencies of these metals. However, the low levels of toxic metals indicate minimal health risks, affirming its safety for medicinal use.
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References
Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 2nd ed. New York: Chapman and Hall; 1984.
Titz A. Medicinal Herbs and Plants – Scope for Diversified and Sustainable Extraction. Bangalore, India; 2004.
Davidson H. Ecological ethnobotany: stumbling toward new practices and paradigms. MASA J. 2000;16:1-13.
Sandhu DS, Heinrich M. The use of health foods, spices and other botanicals in the Sikh community in London. Phytother Res. 2005;19:633–42.
Zaheer S, Inam UK, Habib U, Safdar S, Zahid H, Shahida B, Alam Z, Abdul M. Mineral profile of different medicinal plants and their quantitative analyses collected. Pak J Weed Sci Res. 2014:145-154.
Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol. 2005;4(7):685–688.
Ullah R, et al. Traditional uses of medicinal plants in Darra Adam Khel, N.W.F.P., Pakistan. J Med Plan Res. 2010;4(17):1815–1821.
Suurkuusk G. Validation of the gas chromatographic method for THC, CBD and CBN determination [Master thesis]. Tartu, Estonia: University of Tartu, Faculty of Science and Technology Institute of Chemistry; 2010.
Hanuš LO, Meyer SM, Munoz E, Taglialatela-Scafiti O, Appendino G. Phytocannabinoids: a unified critical inventory. Nat Prod Rep. 2016;33:1357−1392.
Pertwee RGE, editor. Handbook of Cannabis. Oxford: Oxford University Press; 2014.
Hillig KW. Genetic evidence for speciation in Cannabis (Cannabaceae). Gene Res Crop Evo. 2005;52(2):161-180.
Anwar F, Latif S, Ashraf M. Analytical characterization of hemp (Cannabis sativa) seed oil from different agro-ecological zones of Pakistan. J Amer Oil Chem Soc. 2006;83(4):323-329.
Aziz MA, Adnan M, Begum S, Azizullah A, Nazir R, Iram S. A review on the elemental contents of Pakistani medicinal plants: Implications for folk medicines. J Ethnophar. 2016;188:177-192.
Zaidi SH. Existing indigenous medicinal plant resources of Pakistan and their prospects for utilization. Medicinal plants of Pakistan. 2001:53.
Hussain JAL, Khan N, Rehman M. Afr J Biotechnol. 2009;8:2725-2729.
Shinwari ZK. Medicinal plants research in Pakistan. J Med Pl Res. 2010;4(3):161-176.
Okigbo RN, Mmeka EC. An Appraisal of Phytomedicine in Africa. KMITL Sci Tech J. 2006;6(2):83-94.
Miladi S, Damak M. J De La Societe Chimique De Tunisie. 2008;10:101-109.
Duthie GG, Duthie SJ. Nutr Res Rev. 2000;13:79-106.
Sadhir M. Pharmacology of cannabis. J Pain Manag. 2016;9(4):375-379.
Bostwick JM. Blurred Boundaries: The Therapeutics and Politics of Medical Marijuana. Mayo Clin Proc. 2012;87(2):172-186.
Russo EB. History of cannabis and its preparations in saga, science, and sobriquet. Chem Biodivers. 2007;4(8):1614-1648.
Li HL. Hallucinogenic Plants in Chinese Herbals. J Psychedelic Drugs. 1978;10:17-26.
Sahito SR, Kazi TG, Kazi GH, Shar GQ, Memon AN, Pirzado ZA. Comparison of Wet Ashing Method for medicinal plants, Celosia argentea and cubeba officinalis by Atomic Absorption Spectrophotometer. J Chem Soc Pak. 2004;26:135-9.
Heaney RP. Thinking straight about calcium. N Engl J Med. 1993;328(7):503-505.
Food and Nutrition Board, Institute of Medicine. DRI Dietary Reference Intakes for Calcium, Phosphorus, Magnesium, Vitamin D, and Fluoride. Washington, D: National Academy Press; 1997.
Haque MZ, Rauf MA, Jalil MA, Islam MB, Ahsan MA. Studies on the Production of Musabbar Aloe Vera. J Adv Sci Res. 2012;3:51-4.
Thunus L, Lejeune R. Handbook on Metals in Clinical and Analytical Chemistry. New York: Marcel Dekker; 1994.
Rajurkar NS, Pardeshi BM. Analysis of some herbal plants from India used in the control of diabetes mellitus by NAA and AAS techniques. Appl Radiat Isot. 1997;48:1059-62.
Diaconu D, Diaconu R, Navrotescu T. Estimation of heavy metals in medicinal plant and their infusion.
Jagrati A, Ruchi S, Nilima B, Kalpana S. Determination of leaves of uptake of few heavy metals to the leaves of menthaarvensis. Internat J Res Chem Environ. 2012;2:63-7.
Klaassen CD. Casarett and Doull’s Toxicology. 6th ed. New York: McGrawHill; 2001. p. 827-834.
FAO/WHO. Evaluation of certain food additives and contaminants. WHO Technical Report Series No. 837; 1993.