Unveiling the Impact of Nandrolone Decanoate on Kidney and Heart of Rattus Norvegicus
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Abstract
Background: The use of anabolic androgenic steroids (AAS) like Nandrolone Decanoate (ND) for enhancing physical performance and muscle growth is prevalent among athletes and non-athletes. However, the adverse effects of AAS on organ systems, particularly the kidney and heart, have been a growing concern within the medical and scientific community. Previous studies have highlighted the potential nephrotoxic and cardiotoxic effects of these substances, necessitating further research to elucidate the extent of organ damage.
Objective: This study aims to investigate the histopathological effects of ND on the kidney and heart tissues of Rattus norvegicus and to draw comparisons with control groups to assess the severity of organ damage.
Methods: A total of 15 Rattus norvegicus were divided into three groups: a control group, an olive oil group (positive control), and a steroid group (ND treated). The animals were housed under controlled conditions with a 12-hour light/dark cycle at 23±2°C. The steroid and olive oil groups received 50mg/ml intramuscular injections of ND and olive oil, respectively, twice weekly for six weeks. Following treatment, the animals were euthanized, and their kidney and heart tissues were extracted for histological examination. Tissues were fixed in 10% formalin, processed, and stained with Hematoxylin and Eosin (H&E) for microscopic evaluation.
Results: Histological analysis revealed significant alterations in the ND-treated group compared to controls. Kidney tissues from the ND group exhibited tubular dilation, epithelial degeneration, and necrosis with a noticeable increase in Bowman's space and vascular congestion. Quantitatively, tubular atrophy and degeneration were observed in 80% of the ND-treated animals, compared to none in the control and olive oil groups. Heart tissues in the ND group showed evidence of myocardial fibrosis and necrosis, with myocardial fibrosis observed in 70% of the steroid-treated animals, significantly higher than the control groups.
Conclusion: The administration of Nandrolone Decanoate in Rattus norvegicus significantly impacts kidney and heart histology, underscoring the potential health risks associated with AAS use. These findings contribute to the growing body of evidence on the nephrotoxic and cardiotoxic effects of steroids, emphasizing the need for caution and further research in human healthcare to develop safer therapeutic alternatives.
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References
Bahake MS, Yesalis CE. Abuse of anabolic androgenic steroids and related substances in sport and exercise. Curr Opin Pharmacal. 2004;4(6):614-620.
Franke WW, Berendonk B. Hormonal doping and androgenization of athletes: a secret program of the German Democratic Republic government. Clin Chem. 1997;43(7):1262-1279.
Pärssinen M, Seppälä T. Steroid use and long-term health risks in former athletes. Sports Med. 2002;32(2):83-94.
Iriart JAB, Chaves JC, Orleans RGD. Body worship and use of anabolic steroids among bodybuilders. Cad Saude Publica. 2009;25:773-782.
Fuller MG. Anabolic-androgenic steroids use and abuse. Compr Ther. 1993;19(2):69-72.
Lise MLZ, Da Gama e Silva TS, Ferigolo M, Barros HMT. The abuse of anabolic-androgenic steroids in athletics. J Braz Med Assoc. 1999;45(4):364-370.
National Institute on Drug Abuse. Steroid abuse and addiction. NIH Publication No. 06-3721. 2000.
Clarkson PM, Thompson HS. Drugs and sport. Sports Med. 1997;24(6):366-384.
Fahey TD. Anabolic-androgenic steroids: mechanism of action and effects on performance. In: Encyclopedia of Sports Medicine and Science, Internet Society for Sport Science. 1998.
Shahidi NT. A review of the chemistry, biological action, and clinical applications of anabolic-androgenic steroids. Clin Ther. 2001;23(9):1355-1390.
Shabir N, Malik H, Sajjad S, Abbas MN. Effect of nandrolone decanoate, boldenone undecylenate on the renal status of rabbits (Oryctolagus cuniculus). Glob Vet. 2015;14(3):432-438.
Mottram DR, George AJ. Anabolic steroids. Best Pract Res Clin Endocrinol Metab. 2000;14(1):55-69.
Tsika RW, Herrick RE, Baldwin KM. Effect of anabolic steroids on skeletal muscle mass during hind limb suspension. J Appl Physiol. 1987;63(5):2122-2127.
Haupt HA, Rovere GD. Anabolic steroids: a review of the literature. Am J Sports Med. 1984;12(6):469-484.
Evans NA. Current concepts in anabolic-androgenic steroids. Am J Sports Med. 2004;32(2):534-542.
Von Bueren AO, Ma R, Schlumpf M, Lichtensteiger W. Salbutamol exhibits androgenic activity in vitro. Br J Sports Med. 2007;41(12):874-878.
Saha B, Rajadhyaksha GC, Ray SK. Beneficial effects of nandrolone decanoate in wasting associated with HIV. J Indian Med Assoc. 2009;107(5):295-299.
Yersalis CE, Bahrke MS. Anabolic androgenic steroids. Sports Med. 1995;19:326-340.
Kaiser FE, Doe RP. Steroid use in the elderly: Guidelines for avoiding adverse effects. Postgrad Med. 1984;76(1):65-74.
Sullivan ML, Martinez CM, Gennis P, Gallagher EJ. The cardiac toxicity of anabolic steroids. Prog Cardiovasc Dis. 1998;41(1):1-15.
Korkia P, Stimson GV. Indications of prevalence, practice and effects of anabolic steroid use in Great Britain. Int J Sports Med. 1997;18(07):557-562.
Strauss RH, Liggett MT, Lanese RR. Anabolic steroid use and perceived effects in ten weight-trained women athletes. JAMA. 1985;253(19):2871-2873.
Cannavo S, Curto L, Trimarchi F. Exercise-related female reproductive dysfunction. J Endocrinol Invest. 2001;24(10):823-832.
Hassan AF, Kamal MM. Effect of exercise training and anabolic androgenic steroids on hemodynamics, glycogen content, angiogenesis, and apoptosis of cardiac muscle in adult male rats. Int J Health Sci. 2013;7(1):47.
Takahashi M, Tatsugi Y, Kohno T. Endocrinological and pathological effects of anabolic-androgenic steroid in male rats. Endocr J. 2004;51(4):425-434.
McWilliam LJ. Drug-induced renal disease. Curr Diagn Pathol. 2007;13(1):25-31.
Ebeye OA, Ekundina VO, Iosahon RI. Histomorphological effect of nandrolone decanoate on the hepatorenal tissues of adult wistar rat exposed to physical activity. EJPMR. 2016;3(5):285-289.
Choudhury D, Ahmed Z. Drug-associated renal dysfunction and injury. Nat Rev Nephrol. 2006;2(2):80.
Mwaheb MA, Mohammed ARS, Al-Galad GM, Abd-Elgayd AA, Al-hamboly HM. Effect of Nandrolone Decanoate (Anabolic Steroid) on the Liver and Kidney of Male Albino Rats and the Role of Antioxidant (Antox-Silymarin) as Adjuvant Therapy. J Drug Metab Toxicol. 2017;8(1):1-11.
Bento-Silva MT, Martins MDC, Torres-Leal FL, Barros TL, Carvalho ILDNF, Carvalho Filho HA, Almeida FRDC. Effects of administering testosterone undecanoate in rats subjected to physical exercise: effects on the estrous cycle, motor behavior, and morphology of the liver and kidney. Braz J Pharm Sci. 2010;46(1):79-89.
Nieminen MS, Ramo MP, Viitasalo M, Heikkilä P, Karjalainen J, Mäntysaari M, Heikkilä J. Serious cardiovascular side effects of large doses of anabolic steroids in weight lifters. Eur Heart J. 1996;17(10):1576-1583.
Hassan NA, Salem MF, Sayed MAEL. Doping and effects of anabolic androgenic steroids on the heart: histological, ultrastructural and echocardiographic assessment in strength athletes. Hum Exp Toxicol. 2009;28(5):273-283.
Tanno AP, Rosa KT, Cunha TS, Giordano FCL, Calil CM, Guzzoni V, Moura MJCS. Nandrolone and resistance training induce heart remodeling: role of fetal genes and implications for cardiac pathophysiology. Life Sci. 2011;89(17-18):631-637.
Zaugg M, Jamali NZ, Lucchinetti E, Xu W, Alam M, Shafiq SA, Siddiqui MAQ. Anabolic androgenic steroids induce apoptotic cell death in adult rat ventricular myocytes. J Cell Physiol. 2001;187(1):90-95.
Ferenchick GS. Anabolic/androgenic steroid abuse and thrombosis: is there a connection? Med Hypotheses. 1991;35(1):27-31.