Aminoglycoside Resistance in Klebsiella Pneumoniae from Respiratory Specimens: A Phenotypic and Genotypic Analysis: Aminoglycoside Resistance in Klebsiella Pneumoniae

Khaleel Ullah; Sahrish Bhutto; Uzma Riaz; Naveera Mazhar; Hadia Inam; Habiba Saeed; Sabeeha Saddique; Akram Khalid

doi:10.61919/jhrr.v4i3.1392

Authors

Khaleel Ullah Department of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan
Sahrish Bhutto Department of Pediatrics, Dr. Ziauddin Hospital, Karachi, Pakistan
Uzma Riaz Department of Pharmacology, Mohtarma Benazir Bhutto Shaheed Medical College, Mirpur, AJK, Pakistan
Naveera Mazhar School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
Hadia Inam School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
Habiba Saeed School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
Sabeeha Saddique School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
Akram Khalid Faculty of Allied Health Sciences, University of Health Sciences, Lahore, Pakistan

DOI:

https://doi.org/10.61919/jhrr.v4i3.1392

Keywords:

Gram Negative, Facultatively Anaerobic, Capsule, Aminoglycoside Resistance, Biochemical Characteristics, Multi-Drug Resistance

Abstract

Background: Klebsiella Pneumoniae, a Gram-negative bacterium, is increasingly implicated in nosocomial infections, with growing resistance to aminoglycosides due to various mechanisms, including the armA gene.
Objective: This study aimed to compare the phenotypic and genotypic profiles of aminoglycoside-resistant Klebsiella Pneumoniae isolated from respiratory specimens.
Methods: Respiratory specimens (n=80) were collected and processed using standard microbiological methods. Biochemical tests confirmed the isolates as Klebsiella Pneumoniae. DNA was extracted and subjected to PCR to detect the presence of the armA gene. Antibiotic susceptibility testing was performed according to CLSI guidelines. Data were analyzed using SPSS version 25.
Results: Among the 80 isolates, 54% were male, and 46% were female. Biochemical tests showed resistance in 85% of the isolates to at least one class of antibiotics. The armA gene was detected in 86% of the strains, correlating with high-level resistance to gentamicin, tobramycin, and amikacin.
Conclusion: The study highlights significant aminoglycoside resistance in Klebsiella Pneumoniae, with the armA gene playing a critical role. These findings underscore the need for continuous molecular surveillance and alternative therapeutic strategies.

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