Aminoglycoside Resistance in Klebsiella Pneumoniae from Respiratory Specimens: A Phenotypic and Genotypic Analysis Aminoglycoside Resistance in Klebsiella Pneumoniae
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Abstract
Background: Klebsiella Pneumoniae, a Gram-negative bacterium, is increasingly implicated in nosocomial infections, with growing resistance to aminoglycosides due to various mechanisms, including the armA gene.
Objective: This study aimed to compare the phenotypic and genotypic profiles of aminoglycoside-resistant Klebsiella Pneumoniae isolated from respiratory specimens.
Methods: Respiratory specimens (n=80) were collected and processed using standard microbiological methods. Biochemical tests confirmed the isolates as Klebsiella Pneumoniae. DNA was extracted and subjected to PCR to detect the presence of the armA gene. Antibiotic susceptibility testing was performed according to CLSI guidelines. Data were analyzed using SPSS version 25.
Results: Among the 80 isolates, 54% were male, and 46% were female. Biochemical tests showed resistance in 85% of the isolates to at least one class of antibiotics. The armA gene was detected in 86% of the strains, correlating with high-level resistance to gentamicin, tobramycin, and amikacin.
Conclusion: The study highlights significant aminoglycoside resistance in Klebsiella Pneumoniae, with the armA gene playing a critical role. These findings underscore the need for continuous molecular surveillance and alternative therapeutic strategies.
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