Characterization of Aminoglycoside-Resistant Acinetobacter baumannii in Respiratory Specimens: Phenotypic and Molecular Insights

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Saba Safdar
Mahrukh Ghulam Mustafa
Israr Aziz
Ifra Iqrar
Mavara Iqbal


Background: The global rise of antibiotic-resistant bacteria presents a formidable challenge to healthcare systems, with Acinetobacter baumannii standing out as a particularly resilient pathogen in hospital settings. The propensity of this organism to exhibit multidrug resistance complicates treatment protocols and underscores the need for in-depth research into its resistance mechanisms.

Objective: The objective of this study was to characterize the allelotypic and molecular features of aminoglycoside-resistant Acinetobacter baumannii isolates from respiratory specimens and to determine their antibiotic susceptibility profiles to inform better clinical decision-making and treatment approaches.

Methods: This cross-sectional study was conducted at the different labs of various institute of Lahore. A total of 50 respiratory specimens were cultured on selective media. The isolates underwent phenotypic characterization through colony morphology and biochemical tests. Antimicrobial susceptibility was assessed using the disc diffusion method, and molecular analysis was performed using polymerase chain reaction (PCR) to identify the armA gene associated with aminoglycoside resistance. Statistical analysis was executed using SPSS Version 25.

Results: Of the 50 isolates, 54% were from male patients and 46% from female patients. Antibiotic resistance was alarmingly high, with resistance rates of 92% for TZP, 90% for FEP, CAZ, IPM, MEM, 86% for AK, 74% for CN, 54% for TOB, and 48% for DO. The presence of the armA gene was detected in 86% of the isolates, suggesting a link to the high levels of aminoglycoside resistance observed.

Conclusion: The study revealed a high prevalence of multidrug-resistant Acinetobacter baumannii in respiratory specimens, with significant resistance to commonly used antibiotics. These findings highlight the necessity for continuous surveillance of antibiotic resistance patterns and call for innovative approaches to antimicrobial therapy.

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How to Cite
Safdar, S., Mustafa, M. G., Aziz, I., Iqrar, I., & Iqbal , M. (2024). Characterization of Aminoglycoside-Resistant Acinetobacter baumannii in Respiratory Specimens: Phenotypic and Molecular Insights. Journal of Health and Rehabilitation Research, 4(2), 1–8.
Author Biographies

Saba Safdar, Lahore College for Women University LCWU Lahore

BS MLT (KEMU), MS Biotechnology, Department of Biotechnology Lahore College for Women University LCWU Lahore

Mahrukh Ghulam Mustafa, Lahore College for Women University LCWU Lahore Pakistan.

B.Sc. M.Sc. M.Phil. Zoology, Department of Zoology Lahore College for Women University LCWU Lahore

Israr Aziz, Jeju National University Jeju South Korea.

BS MS Micrbiology and Immunology phD, Department of Advanced Convergence Technology and Science, Jeju National University, Jeju, South Korea.

Ifra Iqrar, Government College University Faisalabad Pakistan

Bachelor in Food Science and Technology, Masters in Food Science, Department Food Science and Technology Government College University Faisalabad Pakistan

Mavara Iqbal , University of Veterinary and animal Sciences UVAS Lahore

BS, M.Phil. Microbiology, Department of Microbiology University of Veterinary and animal Sciences UVAS Lahore


Silhavy TJ, Kahne D, Walker S. The bacterial cell envelope. Cold Spring Harb Perspect Biol. 2010;2(5):a000414.

Zhang G, Meredith TC, Kahne D. On the essentiality of lipopolysaccharide to Gram-negative bacteria. Curr Opin Microbiol. 2013;16(6):779–785.

Newton HJ, Ang DK, van Driel IR, Hartland EL. Molecular pathogenesis of infections caused by Legionella pneumophila. Clin Microbiol Rev. 2010;23(2):274–298.

Nocera FP, Attili AR, De Martino L. Acinetobacter baumannii: Its Clinical Significance in Human and Veterinary Medicine. Pathogens. 2021;10(2):127.

Tiku V, Kofoed EM, Yan D, Kang J, Xu M, Reichelt M, et al. Outer membrane vesicles containing OmpA induce mitochondrial fragmentation to promote pathogenesis of Acinetobacter baumannii. Sci Rep. 2021;11(1):618.

Gedefie A, Demsis W, Ashagrie M, Kassa Y, Tesfaye M, Tilahun M, et al. Acinetobacter baumannii Biofilm Formation and Its Role in Disease Pathogenesis: A Review. Infect Drug Resist. 2021;14:3711–3719.

Nimma R, Suravajhala P, Suvarna B, Ahirwar P, Suravajhala R, et al. Identification of potential inhibitors against the DXP pathway enzyme (IspD) from Mycobacterium tuberculosis. Bioinformation. 2023;19(1):030.

Pompilio A, Scribano D, Sarshar M, Di Bonaventura G, Palamara AT, Ambrosi C. Gram-Negative Bacteria Holding Together in a Biofilm: The Acinetobacter baumannii Way. Microorganisms. 2021;9(7):1353.

Avila-Novoa MG, Solís-Velázquez OA, Rangel-López DE, González-Gómez JP, Guerrero-Medina PJ, Gutiérrez-Lomelí M. Biofilm Formation and Detection of Fluoroquinolone- and Carbapenem-Resistant Genes in Multidrug-Resistant Acinetobacter baumannii. Can J Infect Dis Med Microbiol. 2019;2019:3454907.

Krause KM, Serio AW, Kane TR, Connolly LE. Aminoglycosides: An Overview. Cold Spring Harb Perspect Med. 2016;6(6):a027029.

Kyriakidis I, Vasileiou E, Pana ZD, Tragiannidis A. Acinetobacter baumannii Antibiotic Resistance Mechanisms. Pathogens. 2021;10(3):373.

Mora-Ochomogo M, Lohans CT. β-Lactam antibiotic targets and resistance mechanisms: from covalent inhibitors to substrates. RSC Med Chem. 2021;12(10):1623–1639.

Novović K, Jovčić B. Colistin Resistance in Acinetobacter baumannii: Molecular Mechanisms and Epidemiology. Antibiotics (Basel). 2023;12(3):516.

Franco-Duarte R, Černáková L, Kadam S, Kaushik KS, Salehi B, Bevilacqua A, et al. Advances in Chemical and Biological Methods to Identify Microorganisms-From Past to Present. Microorganisms. 2019;7(5):130.

Aliakbarzade K, Farajnia S, Karimi Nik A, Zarei F, Tanomand A. Prevalence of Aminoglycoside Resistance Genes in Acinetobacter baumannii Isolates. Jundishapur J Microbiol. 2014;7(10):e11924.

Vrancianu CO, Gheorghe I, Czobor IB, Chifiriuc MC. Antibiotic Resistance Profiles, Molecular Mechanisms and Innovative Treatment Strategies of Acinetobacter baumannii. Microorganisms. 2020;8(6):935.

Yamin D, Uskoković V, Wakil AM, Goni MD, Shamsuddin SH, Mustafa FH, et al. Current and Future Technologies for the Detection of Antibiotic-Resistant Bacteria. Diagnostics (Basel). 2023;13(20):3246.

Ahmad S, Shakireen N, Ali Khan MS, Mumtaz H, Ahmad W, Shah MH, et al. Prevalence and antimicrobial susceptibility of Acinetobacter spp. in a tertiary care hospital in Peshawar: a cross-sectional study. Ann Med Surg. 2023;85(5):1584–1589.

Madhavan A, Sachu A, Balakrishnan A, Vasudevan A, Balakrishnan S, Vasudevapanicker J. Comparison of PCR and phenotypic methods for the detection of methicillin-resistant Staphylococcus aureus. Iran J Microbiol. 2021;13(1):31–36.

Qu Y, Walker AA, Meng L, Herzig V, Li B. The Predatory Stink Bug Arma custos (Hemiptera: Pentatomidae) Produces a Complex Proteinaceous Venom to Overcome Caterpillar Prey. Biology. 2023 May 9;12(5):691.

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