Assessment of Pharmacological Effects of Methanolic Extract of Perovskia abrotanoides as Anti-Asthmatic and Hepatoprotectant
Pharmacological Effects of P. abrotanoides
DOI:
https://doi.org/10.61919/jhrr.v4i3.1285Keywords:
Perovskia abrotanoides, anti-asthmatic, hepatoprotective, antibacterial, CCl₄-induced hepatotoxicity, histamine-induced bronchospasm, natural remedies, phytochemical screening.Abstract
Background: Perovskia abrotanoides, a plant native to Pakistan, has traditionally been used to treat various ailments, but its pharmacological properties remain underexplored.
Objective: To evaluate the anti-asthmatic, hepatoprotective, antibacterial, and anticancer activities of the methanolic extract of Perovskia abrotanoides.
Methods: The methanolic extract was prepared via maceration and subjected to phytochemical screening. Anti-asthmatic activity was assessed using a histamine-induced bronchospasm model in guinea pigs. Hepatoprotective effects were evaluated in rabbits with CCl₄-induced hepatic damage, measuring liver function tests and conducting histopathological analysis. Antibacterial activity was tested against multiple bacterial strains using the Microplate Alamar Blue Assay, and anticancer activity was evaluated in Hela, 3T3, and MU7 cell lines.
Results: The extract significantly increased pre-convulsive time (58 ± 2.3 seconds) in guinea pigs (P < 0.05), improved liver function markers (P < 0.01), and reduced bilirubin and ALP levels. It showed 81% inhibition against Staphylococcus aureus but no significant anticancer activity.
Conclusion: The methanolic extract of Perovskia abrotanoides demonstrated significant anti-asthmatic, hepatoprotective, and antibacterial properties, with potential for therapeutic use in respiratory and liver conditions.
Downloads
References
Prasathkumar M, Anisha S, Dhrisya C, Becky R, Sadhasivam S. Therapeutic and Pharmacological Efficacy of Selective Indian Medicinal Plants – A Review. Phytomedicine Plus. 2021;1(2):100029.
Khaliq S, Volk F-J, Frahm AW. Phytochemical Investigation of Perovskia Abrotanoides. Planta Medica. 2007;73(01):77-83.
Ashraf SN, Zubair M, Rizwan K, Tareen RB, Rasool N, Zia-Ul-Haq M, et al. Compositional Studies and Biological Activities of Perovskia Abrotanoides Kar. Oils. Biological Research. 2014;47(1):12.
Aziz Q, Maimoona Q, Ziaullah, Maryum M, Arooj MA, Kashif I, et al. Comparative Assessment of Compounds in Lawsonia Inermis L. - A Potent Antileishmanial Plant. Advances in Pharmacology and Clinical Trials. 2023;8(4):000230.
Beikmohammadi M. The Evaluation of Medicinal Properties of Perovskia Abrotanoides Karel. 2012.
Arabi F, Moharramipour S, Sefidkon F. Chemical Composition and Insecticidal Activity of Essential Oil From Perovskia Abrotanoides (Lamiaceae) Against Sitophilus Oryzae (Coleoptera: Curculionidae) and Tribolium Castaneum (Coleoptera: Tenebrionidae). International Journal of Tropical Insect Science. 2008;28(3):144-50.
Alcaraz MJ, Jimenez MJ, Gallego R, Cuttis J, Navarro C, Villar A. Flavonoids as Anti-Inflammatory Agents: Structure-Activity Relationship. Journal of Drug Research. 1989; 21:51-6.
Iqbal K, Aziz Q, Fatima A, Sheikh MA, Ahmed T. Comparative Study on Antileishmanial and Cytotoxic Activity of Lawsonia Inermis Bark and Aloe Vera Leaves. International Journal of Biology Pharmacy and Allied Sciences. 2016;5(6):1490-1500.
Gokhale A, Saraf M. Bronchoprotective Effect of Ethanolic Extract of Tephrosia Purpurea In Vivo. Indian Drugs-Bombay. 2000;37(7):346-7.
Iqbal K, Aziz Q, Sheikh MA. Assessment of In Vitro and In Vivo Effect of Quercetin 3-Glucoside, Oxyresveratrol and Quercetin O-Hexoside Against Leishmania Tropica. Brazilian Journal of Pharmaceutical Sciences. 2022;58.
Yasmin S, Akram KM, Anwar K. Screening of Aerial Parts of Abutilon Bidentatum for Hepatoprotective Activity in Rabbits. 2011.
Feroz Z, Khan RA, Afroz S. Cumulative Toxicities on Lipid Profile and Glucose Following Administration of Anti-Epileptic, Anti-Hypertensive, Anti-Diabetic, and Anti-Arrhythmic Drugs. Pak J Pharm Sci. 2011;24(1):47-51.
Ahmad M, Muhammed S, Jahan N, Jan SU. Anti-Dermatitis, Anxiolytic and Analgesic Effects of Rhazya Stricta From Balochistan. Pakistan Journal of Pharmaceutical Sciences. 2014;27(3):481-7.
Olusola LA, Matthew OA, Oluwatosin AA. Comparative Study on the Effects of Aqueous Extracts of Viscum Album (Mistletoe) From Three Host Plants on Hematological Parameters in Albino Rats. African Health Sciences. 2015;15(2):606-12.
Alvi AM, Al Kury LT, Alattar A, Ullah I, Muhammad AJ, Alshaman R, et al. Carveol Attenuates Seizure Severity and Neuroinflammation in Pentylenetetrazole-Kindled Epileptic Rats by Regulating the Nrf2 Signaling Pathway. Oxidative Medicine and Cellular Longevity. 2021;2021:9966663.
Ashraf S, N, Zubair M, Rizwan K, Tareen RB, Rasool N, Zia-Ul-Haq M, et al. Compositional Studies and Biological Activities of Perovskia Abrotanoides Kar. Oils. Biological Research. 2014;47(1):12.
Maleki SJ, Crespo JF, Cabanillas B. Anti-Inflammatory Effects of Flavonoids. Food Chemistry. 2019;299:125124.
Maryam F, Vahid P, Amirahmadi A, Salimi F. Perovskia Abrotanoides Kar. As A Promising Source of Antimicrobial Compounds Against Foodborne Pathogens. Medical Laboratory Journal. 2023;17(3):45-54.
Ur Rahman S, Adhikari A, Ismail M, Raza Shah M, Khurram M, Shahid M, et al. Beneficial Effects of Trillium Govanianum Rhizomes in Pain and Inflammation. Molecules. 2016;21(8):1095.
Sitarek P, Merecz-Sadowska A, Śliwiński T, Zajdel R, Kowalczyk T. An In Vitro Evaluation of the Molecular Mechanisms of Action of Medical Plants From the Lamiaceae Family As Effective Sources of Active Compounds Against Human Cancer Cell Lines. Cancers. 2020;12(10):2957.
Porchezhian E, Ansari S. Hepatoprotective Activity of Abutilon Indicum on Experimental Liver Damage in Rats. Phytomedicine. 2005;12(1-2):62-4.
Jung HA, Abdul QA, Byun JS, Joung EJ, Gwon WG, Lee MS, et al. Protective Effects of Flavonoids Isolated From Korean Milk Thistle Cirsium Japonicum var. Maackii (Maxim.) Matsum on Tert-Butyl Hydroperoxide-Induced Hepatotoxicity in HepG2 Cells. Journal of Ethnopharmacology. 2017;209:62-72.
Prabhavathi O, Yuvarajan R, Natarajan D. Mosquitocidal Properties of Ocimum Canum Sims (Lamiaceae) Leaf Extracts Against Dengue Vector Aedes Aegypti L.(Diptera: Culicidae). Journal of Entomological and Acarological Research. 2016;48(3):345-54.
Sheikh A, Yeasmin F, Agarwal S, Rahman M, Islam K, Hossain E, et al. Protective Effects of Moringa Oleifera Lam. Leaves Against Arsenic–Induced Toxicity in Mice. Asian Pacific Journal of Tropical Biomedicine. 2014;4.
Zhu PC, Shen J, Qian RY, Xu J, Liu C, Hu WM, et al. Effect of Tanshinone IIA for Myocardial Ischemia/Reperfusion Injury in Animal Model: Preclinical Evidence and Possible Mechanisms. Frontiers in Pharmacology. 2023;14:1165212.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Marvi, Maimoona Qadir, Ziaullah, Maryum Mumshad, Arooj Mohsin Alvi, Kashif Iqbal
This work is licensed under a Creative Commons Attribution 4.0 International License.
